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lox stop lox reporter cassette called xr1  (Addgene inc)


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    Structured Review

    Addgene inc lox stop lox reporter cassette called xr1
    Lox Stop Lox Reporter Cassette Called Xr1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lox+stop+lox+cassette/XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP+(Plasmid+%23129111)/bio_rxiv__2024__12__03__626573-228-2-6
    Average 92 stars, based on 1 article reviews
    lox stop lox reporter cassette called xr1 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Loss of Quaking RNA binding protein disrupts the expression of genes associated with astrocyte maturation in mouse brain
    Article Snippet: Mice were maintained in standard housing conditions with food and water provided ad libitum , and crossed at each generation to wildtype C57BL/6J mice from Jackson labs. .. To generate a Cre-dependent Cas9-expressing mouse line, we engineered Cas9 into a targeting vector containing Rosa26 homology arms (~1 kb, PCR’s from genome), flanking a CAG enhancer, Lox-Stop-Lox cassette (adapted from the Allen AI9 constructs, Addgene Plasmid #22799), with 3xFlag-NLS-Cas9-NLS cloned downstream of the stop cassette. ..

    Article Title: CRISPR-TRAPSeq identifies the QKI RNA binding protein as important for astrocytic maturation and control of thalamocortical synapses
    Article Snippet: Mice were maintained in standard housing conditions with food and water provided ad libitum , and crossed at each generation to wildtype C57BL/6J mice from Jackson labs. .. To generate a Cre-dependent Cas9-expressing mouse line, we engineered Cas9 into a targeting vector containing Rosa26 homology arms (∼1 kb, PCR’s from genome), flanking a CAG enhancer, Lox Stop Lox cassette (adapted from the Allen AI9 constructs, Addgene Plasmid #22799), with 3xFlag-NLS-Cas9-NLS cloned downstream of the stop cassette. ..

    Polymerase Chain Reaction:

    Article Title: Loss of Quaking RNA binding protein disrupts the expression of genes associated with astrocyte maturation in mouse brain
    Article Snippet: Mice were maintained in standard housing conditions with food and water provided ad libitum , and crossed at each generation to wildtype C57BL/6J mice from Jackson labs. .. To generate a Cre-dependent Cas9-expressing mouse line, we engineered Cas9 into a targeting vector containing Rosa26 homology arms (~1 kb, PCR’s from genome), flanking a CAG enhancer, Lox-Stop-Lox cassette (adapted from the Allen AI9 constructs, Addgene Plasmid #22799), with 3xFlag-NLS-Cas9-NLS cloned downstream of the stop cassette. ..

    Article Title: CRISPR-TRAPSeq identifies the QKI RNA binding protein as important for astrocytic maturation and control of thalamocortical synapses
    Article Snippet: Mice were maintained in standard housing conditions with food and water provided ad libitum , and crossed at each generation to wildtype C57BL/6J mice from Jackson labs. .. To generate a Cre-dependent Cas9-expressing mouse line, we engineered Cas9 into a targeting vector containing Rosa26 homology arms (∼1 kb, PCR’s from genome), flanking a CAG enhancer, Lox Stop Lox cassette (adapted from the Allen AI9 constructs, Addgene Plasmid #22799), with 3xFlag-NLS-Cas9-NLS cloned downstream of the stop cassette. ..

    Construct:

    Article Title: Loss of Quaking RNA binding protein disrupts the expression of genes associated with astrocyte maturation in mouse brain
    Article Snippet: Mice were maintained in standard housing conditions with food and water provided ad libitum , and crossed at each generation to wildtype C57BL/6J mice from Jackson labs. .. To generate a Cre-dependent Cas9-expressing mouse line, we engineered Cas9 into a targeting vector containing Rosa26 homology arms (~1 kb, PCR’s from genome), flanking a CAG enhancer, Lox-Stop-Lox cassette (adapted from the Allen AI9 constructs, Addgene Plasmid #22799), with 3xFlag-NLS-Cas9-NLS cloned downstream of the stop cassette. ..

    Article Title: CRISPR-TRAPSeq identifies the QKI RNA binding protein as important for astrocytic maturation and control of thalamocortical synapses
    Article Snippet: Mice were maintained in standard housing conditions with food and water provided ad libitum , and crossed at each generation to wildtype C57BL/6J mice from Jackson labs. .. To generate a Cre-dependent Cas9-expressing mouse line, we engineered Cas9 into a targeting vector containing Rosa26 homology arms (∼1 kb, PCR’s from genome), flanking a CAG enhancer, Lox Stop Lox cassette (adapted from the Allen AI9 constructs, Addgene Plasmid #22799), with 3xFlag-NLS-Cas9-NLS cloned downstream of the stop cassette. ..

    Clone Assay:

    Article Title: Loss of Quaking RNA binding protein disrupts the expression of genes associated with astrocyte maturation in mouse brain
    Article Snippet: Mice were maintained in standard housing conditions with food and water provided ad libitum , and crossed at each generation to wildtype C57BL/6J mice from Jackson labs. .. To generate a Cre-dependent Cas9-expressing mouse line, we engineered Cas9 into a targeting vector containing Rosa26 homology arms (~1 kb, PCR’s from genome), flanking a CAG enhancer, Lox-Stop-Lox cassette (adapted from the Allen AI9 constructs, Addgene Plasmid #22799), with 3xFlag-NLS-Cas9-NLS cloned downstream of the stop cassette. ..

    Article Title: CRISPR-TRAPSeq identifies the QKI RNA binding protein as important for astrocytic maturation and control of thalamocortical synapses
    Article Snippet: Mice were maintained in standard housing conditions with food and water provided ad libitum , and crossed at each generation to wildtype C57BL/6J mice from Jackson labs. .. To generate a Cre-dependent Cas9-expressing mouse line, we engineered Cas9 into a targeting vector containing Rosa26 homology arms (∼1 kb, PCR’s from genome), flanking a CAG enhancer, Lox Stop Lox cassette (adapted from the Allen AI9 constructs, Addgene Plasmid #22799), with 3xFlag-NLS-Cas9-NLS cloned downstream of the stop cassette. ..



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